Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR*□S
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Physiological adaptation of the bacterium Lactococcus lactis in response to the production of human CFTR.
Biochemical and biophysical characterization of CFTR (the cystic fibrosis transmembrane conductance regulator) is thwarted by difficulties to obtain sufficient quantities of correctly folded and functional protein. Here we have produced human CFTR in the prokaryotic expression host Lactococcus lactis. The full-length protein was detected in the membrane of the bacterium, but the yields were too...
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تاریخ انتشار 2017